Stabilized, Non-Fouling Transmission Electron Microscopy Grid Coatings for the Selective Capture of His-Tag T7 Virus and His-Tag Gro EL from Cell Lysates
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چکیده
منابع مشابه
Synthetics of NiFe2O4 nanoparticles for recombinant His-tag protein purification
Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...
متن کاملSynthetics of NiFe2O4 nanoparticles for recombinant His-tag protein purification
Recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. Genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. Differed methods for protein purification are categorized, based on cast, quality, Easy work and side effect of protein. In this article, we are investigating his His-tag protein pu...
متن کاملsynthetics of nife2o4 nanoparticles for recombinant his-tag protein purification
recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...
متن کاملRecombinant production and affinity purification of the FraC pore forming toxin using hexa-His tag and pET expression cassette
Objective(s): A newly-introduced protein toxin from a sea anemone, namely fragaceatoxin C is a protein with molecular weight of 20 kDa and pore-forming capability against cell membranes has recently grasped great attentions for its function. In this study, its coding sequence cloned as a fusion protein with His-tag for simple production and rapid purification. Materials and Methods: After PCR a...
متن کاملIn-cell covalent labeling of reactive His-tag fused proteins.
A new method for in-cell protein labeling was developed. This method employed a binding-induced nucleophilic reaction between the Cys-appended His-tag and the Ni(II)-NTA containing an α-chloroacetamide. Using this method, not only labeling of His-tag fused proteins but also the detection of a protein-protein interaction was achieved inside living cells.
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2015
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2014.11.3361